Measuring Ketones: The Difference Between Testing Breath and Blood

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The ketogenic diet has been demonstrated to have a wide variety of benefits spanning multiple populations including the treatment of epilepsy, type-2 diabetes, neurological disorders, weight loss, endurance performance and even certain types of cancer treatments (1-5). The staple of a ketogenic diet is a rise of ketone bodies which happens following a prolonged period of carbohydrate or caloric restriction (i.e, fasting). Ketone bodies are produced in the liver as an alternative fuel source to carbohydrates and measuring ketones is a way to determine the degree of ketosis someone is in.

Why Measuring Ketones Matters

Being able to frequently and accurately measure the level of ketosis can be important for those following the ketogenic diet for clinical purposes as well as for overall health and performance. Due to individual variability in response to different types of food, having frequent feedback will aid individuals towards improving their understanding of how their body responds while increasing motivation and adherence.

This need has led researchers and practitioners to be on the look-out for fast and accurate methods to assess ketone levels. Traditionally, ketone levels have been assessed via urine strips and while this method may be easiest, it is not the most practical.  This method measures the excretion of ketones and wasn’t intended for people following a ketogenic diet; rather it was intended for those suffering from conditions such as diabetic ketoacidosis.  This is because urine strips test for unused acetoacetate rather than the main ketone that our body does use, B-hydroxybutyrate.

In the ketogenic space, measuring the blood B-hydroxybutyrate concentration is the standard protocol. While this method has been shown to be more accurate than urine tests, these blood assessments may be troublesome for children and elderly, and could become expensive (requires a constant supply of blood strips), and not as practical outside of a clinical setting.

Recently, researchers have identified breath acetone assessments as a practical and accurate method to determine level of ketosis (6).

What is Breath Acetone?

When following a ketogenic diet, acetyl-CoA is produced in the liver from the breakdown of fat and is used to produce acetoacetate, one of three ketone bodies.  From there, acetoacetate can be converted to the other two “ketone bodies”, b-hydroxybutyrate and acetone.  While b-hydroxybutyrate is tested via blood meters, acetone actually diffuses into the lungs and can be measured by testing exhaled breath (7)! Acetone is a byproduct of fat metabolism and is present in the breath of all humans but in different concentrations.

In healthy individuals, resting acetone levels may range from 0.5 to 2.0 ppm. Individuals following the ketogenic diet can see levels of acetone up to 40 ppm. Individuals undergoing a prolonged fast may see elevated levels up to 170 ppm. Lastly, uncontrolled diabetes and subsequent ketoacidosis may lead to acetone levels of 1250 ppm (8).

It is also interesting to note that as acetone is a byproduct of fat metabolism, it can also be used to measure the rate of fat loss (9).

More specific for those following a ketogenic diet, to date, six studies have compared breath acetone levels with blood b-hydroxybutyrate levels and have found a strong correlation (R2= 0.77) (8). One study in particular directly compared blood, breath, and urine samples of 12 healthy individuals undergoing an experimental protocol designed to induce a state of ketosis. The results demonstrated that plasma acetoacetate was best predicted by breath acetone (6). Therefore, it appears that breath acetone assessments are a fast and accurate way to test for the degree of ketosis.

Wrapping it up: Measuring Ketones (Blood VS. Breath)

While blood assessments are a great way to measure ketosis, breath acetone assessments also appear to be a fast and accurate measure to determine the degree of ketosis. This is not to say that blood assessments should lose any of their value as they are still extremely valuable and should be taken in conjunction with breath assessments. However, the major benefit to breath assessments is the practicality of breath ketone levels as it can be assessed frequently with no invasive procedures helping individuals maximize their ketogenic diets!

Heads Up Health lets you track all types of ketone measurements – urine, breath and blood – so you can choose the method that works best for your lifestyle and budget. Additionally, the LEVL and Ketonix breath ketone meters can be electronically linked to your Heads Up Health account so readings will be synchronized automatically and can be tracked alongside all of your other vital health data. Start your free 30-day trial today!

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References

1.)   Neal, E. G., Chaffe, H., Schwartz, R. H., Lawson, M. S., Edwards, N., Fitzsimmons, G., … & Cross, J. H. (2008). The ketogenic diet for the treatment of childhood epilepsy: a randomised controlled trial. The Lancet Neurology, 7(6), 500-506.

2.)   Westman, E. C., Yancy, W. S., Mavropoulos, J. C., Marquart, M., & McDuffie, J. R. (2008). The effect of a low-carbohydrate, ketogenic diet versus a low-glycemic index diet on glycemic control in type 2 diabetes mellitus. Nutrition & metabolism, 5(1), 36.

3.)   Gasior, M., Rogawski, M. A., & Hartman, A. L. (2006). Neuroprotective and disease-modifying effects of the ketogenic diet. Behavioural pharmacology, 17(5-6), 431.

4.)   Johnstone, A. M., Horgan, G. W., Murison, S. D., Bremner, D. M., & Lobley, G. E. (2008). Effects of a high-protein ketogenic diet on hunger, appetite, and weight loss in obese men feeding ad libitum–. The American journal of clinical nutrition, 87(1), 44-55.

5.)   Zhou, W., Mukherjee, P., Kiebish, M. A., Markis, W. T., Mantis, J. G., & Seyfried, T. N. (2007). The calorically restricted ketogenic diet, an effective alternative therapy for malignant brain cancer. Nutrition & metabolism, 4(1), 5.

6.)   Musa-Veloso, K., Likhodii, S. S., & Cunnane, S. C. (2002). Breath acetone is a reliable indicator of ketosis in adults consuming ketogenic meals. The American journal of clinical nutrition, 76(1), 65-70.

7.)   Kalapos, M. P. (2003). On the mammalian acetone metabolism: from chemistry to clinical implications. Biochimica et Biophysica Acta (BBA)-General Subjects, 1621(2), 122-139.

8.)   Anderson, J. C. (2015). Measuring breath acetone for monitoring fat loss. Obesity, 23(12), 2327-2334.

9.)   Freund, G. (1965). The calorie deficiency hypothesis of ketogenesis tested in man. Metabolism-Clinical and Experimental, 14(9), 985-990.

About the Author


Chris Irvin, MS is known as The Ketologist and has spent his research background studying ketosis in both human and animal populations for general health, human performance, and therapeutics. Chris now focuses his efforts towards educating the ketogenic community on the benefits of a ketogenic diet and how to practically apply it to your lifestyle. Chris blogs on his website theketologist.com where he covers all aspects of ketogenic and low carb dieting.
 
 

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